生物物理与神经生物学前沿学术报告

       Frontiers in Biophysics and Neurobiology 

报告题目: Neurotropic Herpesviruses and the Derivate Anterograde neuronal circuit Tracers

报告人:　 Min Hua Luo, Ph.D

                    Professor of Wuhan Institute of Virology Chinese Academy of Sciences

时间：　  2018年1月8日（周一），下午：14:00-15:00

地点：　　西区生物楼531会议室

主办单位：中国科学院脑功能与脑疾病重点实验室

中国科学技术大学生命科学学院

合肥微尺度物质科学国家实验室集成影像中心

报告简介：

Herpes simplex virus type 1 strain 129 (H129) represented  a potential anterograde neuronal circuit tracing tool, which complements  the existing retrograde tracers. However, the current H129 derived  tracers are multisynaptic, neither bright enough to label the details of  neurons nor capable of determining direct projection targets  as a monosynaptic tracer. Here we introduce an improved multisynaptic  anterograde tracer H129-G4 with strong labeling intensity, and a novel  mono-synaptic anterograde tracer H129-ΔTK-tdT. H129-G4 is capable of  transmitting through multiple synapses, labeling the neurons by green  florescent protein, and visualizing the morphological details of the  labeled neurons. H129-ΔTK-tdT neither replicates nor spreads in neurons  alone, but transmits to and labels the postsynaptic neurons with  tdTomato in the presence of complementary expressed TK from a helper  virus. H129-ΔTK-tdT is also capable to map the direct projectome of the  specific neuron type in the given brain regions in Cre transgenic mice.  The H129-ΔTK-tdT anterograde monosynaptic tracing system offers a useful  tool for mapping the direct output in neuronal circuitry. H129-G4 is an  anterograde multisynaptic tracer with a labeling signal strong enough  to display the details of neuron morphology.

How to improve the tracers, how viral particles traffic in axon and how to display the tracing efficiency are undergoing study.